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The triangular culture flask is composed of a bottle body and a bottle cap.. The unique bottle bottom design makes it easier for pipettes or cell scrapers to reach the corner of the bottle, increasing the convenience of cell culture operations. and stability. The common sizes of triangular shake flasks are 125ml, 250ml, 500ml and 1000ml. In order to observe the capacity of the medium and grasp the growth state of the cells, a scale will be printed on the bottle body. Cell culture needs to be carried out in a sterile environment. Therefore, the Erlenmeyer flask will undergo special sterilization treatment before being put into use to achieve the effect of no DNase, no RNase, and no animal-derived ingredients, providing good conditions for cell growth. surroundings.Cells Grow Slowly in Erlenmeyer Flask and Solution What causes slow growth of cells in cell shaker flasks Cells are very sensitive to the growth environment. When culturing cells, we sometimes encounter slow cell growth. What is the reason? There are many reasons for the slow growth of cells in the cell shake flask, mainly for the following reasons: 1. The cells need to be re-adapted due to the change of different culture medium or serum. 2. The reagents are improperly stored, and some necessary components for cell growth such as glutamine or growth factors in the culture medium are exhausted or lacking or have been destroyed. 3. There is a small amount of bacterial or fungal contamination in the culture in the cell shaker. 4. The initial concentration of inoculated cells is too low. 5. Cells have aged. 6. Mycoplasma contamination Suggested solution: 1. Compare the composition of the new medium and the original medium, and compare the new serum and the old serum to support cell growth experiments. Allow cells to gradually adapt to the new medium. 2. Change into freshly prepared culture medium, or add glutamine and growth factors. 3. Incubate with antibiotic-free medium and replace the culture if contamination is found. The culture medium should be stored at 2-8°C in the dark. Serum-containing complete medium is stored at 2-8°C and used up within 2 weeks. 4. Increase the starting concentration of inoculated cells. 5. Replace with new seeded cells. 6. Isolate the culture and detect mycoplasma. Clean the stand and incubator. If mycoplasma contamination is found, replace with a new culture.Erlenmeyer Shake Flask Feature The erlenmeyer flask, also known as the triangular shake flask, is mainly suitable for the cultivation of insect cell lines with high oxygen requirements. Compared with consumables such as cell factories and cell spinner flasks, the cell culture area is small and it is an economical cell culture tool. . The flask body is made of polycarbonate (PC) or PETG material. The unique triangular shape design makes it easier for pipettes or cell scrapers to reach the corner of the flask, making cell culture operations more convenient. The bottle cap is made of high-strength HDPE material, which is divided into a sealing cap and a breathable cap. The sealing cap is used for the sealed culture of gas and liquid. The vent cap is equipped with a hydrophobic filter membrane on the top of the bottle cap. It prevents the entry and exit of microorganisms, prevents pollution, and ensures gas exchange, so that cells or bacteria grow well.